Why does insect rna look degraded

Readers on mendeley Mendeley. Summary Twitter Dimensions citations. You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.

Click here to find out more. View on publisher site Alert me about new mentions. Twitter Demographics The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled. Mendeley readers. The data shown below were compiled from readership statistics for Mendeley readers of this research output. Click here to see the associated Mendeley record. Attention Score in Context. Their molecular size suggests dissociation of their 28S rRNA despite being in the same order with aphids.

This observation was further strengthened by comparative analysis of possible secondary structures of variable regions that aligned to B. Structures predicted from T. Nevertheless, phylogenetic analysis of their sequences Figure 3 did not support this observation. Instead, divergence was observed between their 28S rRNA sequences.

On the other hand, structures of variable regions derived from G. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article of the Year Award: Outstanding research contributions of , as selected by our Chief Editors. Read the winning articles. Journal overview. Special Issues. Academic Editor: Ben Berkhout. Received 28 Aug Revised 04 Nov Accepted 19 Jan Published 12 Feb Materials and Methods 2. Insect Sampling Representative insects for the five orders were obtained from insect colonies maintained at the icipe insectaries and screen houses. Denaturing Agarose Gel Electrophoresis A 1.

Nondenaturing Agarose Gel Electrophoresis A 1. Table 1. Nanodrop readings for RNA samples: Brevicoryne brassicae , Thrips tabaci , whiteflies Aleyrodidae , Anopheles arabiensis , Glossina fuscipes fuscipes , Dacus bivittatus , Chilo partellus , Bombyx mori , Apis mellifera , and Fopius arisanus.

Table 2. Figure 1. Gel images showing the banding profiles of the various insect RNA sampled. On the other hand, b shows RNA appearance on 1. Figure 2. Multiple alignment of gap region of B. Dots indicate gaps introduced during alignment to maximize homology. Figure 3. Amidpoint rooted maximum likelihood generated from 28S rRNA sequences for representative species in five major insect orders.

The cladogram was generated using PhyML with bootstrap replicates. Node labels show support bootstrap values. Figure 4. References A. Kazantsev and N. Aranda, D. Apis invertebrate Apidae. Citation Type. Has PDF. Publication Type. More Filters. View 2 excerpts, cites background and results. View 1 excerpt, cites background.

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